DISTINCTIVE SIRE1 RETROTRANSPOSON PATTERNS ON BARLEY CHROMOSOMES?
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Research Article
VOLUME: 22 ISSUE: 1
P: 9 - 15
April 2021

DISTINCTIVE SIRE1 RETROTRANSPOSON PATTERNS ON BARLEY CHROMOSOMES?

Trakya Univ J Nat Sci 2021;22(1):9-15
1. İstinye University, Department of Molecular Biology and Genetics, İstanbul, TURKEY
2. İstanbul University, Faculty of Sciences, Department of Molecular Biology and Genetics, İstanbul, TURKEY
No information available.
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Received Date: 24.07.2020
Accepted Date: 09.10.2020
Online Date: 15.04.2021
Publish Date: 15.04.2021
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Abstract

SIRE1 is an active and relatively high copy-number retroelement belongs to the Tyl/Copia long terminal repeat (LTR) retrotransposon superfamily. Distinctive SIRE1 elements (ENV and GAG) distributions in barley genome were observed by using fluorescent in situ hybridization (FISH). We performed PCR to obtain tetramethylrhodamine-dUTP (TRITC)-labelled probes. Localizations of SIRE1 ENV and GAG domains were demonstrated under confocal microscope on Hordeum vulgare L. cv. Hasat root preparations. Our results revealed the distributions of SIRE1 elements ENV and GAG in barley genome. These results may provide to uncover the organization of SIRE retrotransposon pattern in barley genome.

Keywords:
Fluorescence in situ hybridization, Retrotransposon, SIRE1, Barley, ENV, GAG