Abstract

Vancomycin production as well as extracellular glucose and glycerol level and pH level of A.orientalis growth in different glucose (5-20 g/L) and glycerol (2.5-20 g/L) concentrations of the medium was investigated during the incubation period. A. orientalis growth rate increased with the increases in glucose and glycerol concentration while pH levels of the growth medium decreased significantly. In addition, a positive correlation was determined between consumption time of extracellular glucose levels and glucose concentration in the culture medium. Similar results were also determined in glycerol supplemented medium. As a glycopeptid antibiotic, vancomycin production of A. orientalis increased with the increases in glucose and glycerol concentrations up to 15 and 10 g/L, respectively. In addition, substitution of glycerol with glucose significantly affected vancomycin antibiotic productions whereas the growth rate was close to that of the each other.